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利用基因枪转化系统，将含有由ＣａＭＶ３５Ｓ启动子启动的ｂａｒ基因的转化质粒ｐＣＢ１导入水稻幼胚，经筛选、再生得到３株抗除草剂Ｂａｓｔａ的转基因植株。经ＰＣＲ及Ｓｏｕｔｈｅｒｎ分析，在转基因植株基因组中均检测到了ｂａｒ基因的整合；经ＲＮＡ点杂交分析，检测到了ｂａｒ基因在转基因植株中ＲＮＡ水平的表达。在转基因植株ＪＹ１１９－２的总计３２１株Ｔ１代自交后代中，有２７４株表现出除草剂抗性，４７株敏感。从该２７４株抗性植株中随机选取８株进行Ｓｏｕｔｈｅｒｎ分析，结果均检测到了ｂａｒ基因的整合，表明ｂａｒ基因已遗传到了Ｔ１代植株中
Using the gene gun transformation system, the transformed plasmid pCB1 containing the bar gene initiated by the CaMV35S promoter was introduced into rice immature embryos, and three transgenic plants resistant to the herbicide Basta were obtained after being screened and regenerated. The integration of bar gene was detected in the genome of transgenic plants by PCR and Southern analysis. The RNA expression of bar gene in transgenic plants was detected by RNA dot blot analysis. Of the 321 T1 selfed offspring of transgenic plants JY119-2, 274 showed herbicide resistance and 47 were sensitive. Eight of the 274 resistant plants were randomly selected for Southern analysis and the bar gene integration was detected, indicating that the bar gene had been inherited to T1 plants