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This experiment was performed to investigate the calcification of the cryopreserved aortic and pulmonary homografts quantitively after implantation. Methods: In operation the heterotopic implantation of homograft conduits into the abdominal aorta was adopted. The calcification of the homograft conduits was determined by SX micro electronic probe analysis 8 wk after implantation. Results: Before implantation the calcium content of aortic homografts [(0.24±0.05) μg/mg] was significantly higher than that of pulmonary homograft [(0.13 ±0.02) μg/mg,P<0.01]. After implantation the calcium content ofarotic homografts [(0.53±0.09) μg/mg] was significantly higher than that before implantation [(0.24±0.05) μg/mg], with an increase by (123.4±53.0)% (P<0.01), and so was that of pulmonary homografts from [(0.13±0.02) to (0.21±0.30) μg/mg], with an increase by (63.8±35.8)%(P<0.01). And the calcium content of aortic homograft [(0.53±0.09) μg/mg] was still significantly higher than that of pulmonary homograft [(0.21±0.03) μg/mg, P<0.01 ]. Conclusion: In homograft conduit implantation the aortic homografts are more easily calcified than pulmonary homografts. The Micro electronic probe technique has a great significance in element component analysis of biological preparation.