采用聚丙烯酰胺凝胶电泳法，研究了柑桔属４个种的６个品种，两个营养系芽变品系和１个杂交后代的成熟春梢的叶片酯酶同工酶酶谱和蛋白质电泳带型。根据酶谱及带型的异同，探讨其彼此的亲缘关系，鉴别芽变品系和杂种。结果表明，酯酶同工酶酶带清晰，种间酶带差异明显，种内不明显。蛋白质电泳带无拖尾现象，清晰而窄，种间和种内不同品种间谱带差异明显；芽变品系具有原品种所有带型，并出现新的谱带；种间杂种带型复杂，谱带条数多。不仅具有双亲所具有的谱带，又不完全表现为两亲本谱带的互补型，亲本所具有的个别谱带在子代中消失，而且出现了双亲所不具有的杂种谱带。柑桔叶片酯酶同工酶分析，可作为探测基因差异和遗传关系的一种手段，其酶谱可作为鉴别种以上遗传性状的指标，叶片蛋白质电泳，可用来进行种或品种区分，有效地鉴别芽变品系和杂种 Polyacrylamide gel electrophoresis was used to study the changes of esterase isozymes and protein electrophoresis in six varieties of four species of citrus, two vegetative lines and one of the hybrids Belt type. According to the similarities and differences of zymogram and banding patterns, the relationship between them was explored, and the strains and hybrids of budding mutants were identified. The results showed that the esterase isozymes with a clear band, significant differences between species of enzyme, species is not obvious. There was no trailing phenomenon in the protein electrophoresis bands, which was clear and narrow. The bands between different species within and between species were obviously different. The mutant strains had all the bands of the original species and appeared new bands. The interspecific hybrid bands were complex, With a large number of strips. Not only has the parents have the band, but also does not fully show the amphiphilic complementarity of the band, the parent has the individual bands disappear in the offspring, but also appeared in the parents do not have the hybrid bands. The analysis of esterase isozymes in leaves of Citrus can be used as a means to detect the genetic difference and genetic relationship. The zymogram can be used as an index to identify the above genetic traits, and leaf protein electrophoresis can be used to distinguish species or breeds effectively Identify buds and hybrids