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用Heidenhain氏铁苏木素进行线粒体染色,无论中外技术书籍均有介绍,称为上乘。但不是如书所说,每染必成,而且叙述也不够详细,为了今后在工作中更能正确使用该法,尤其对于初学者易于掌握,我们作了多次试验,得出一些不成熟的体会与收获。现将实际操作列下。 1.取材与固定取幼小活泼之小白鼠一只,用手敲延髓致死,立即剖腹取小肠、胰、肾等组织数小块,每块约2毫米长,迅速固定于Regaud氏液,该固定液与原方略有不同: 3%重铬酸钾 30ml(原40ml) 中性福马林(碳酸钙中和)10ml 固定4日后,继以3%重铬酸钾铬化8天,均在冰箱操作,每日换新液,并每发现液体变黑,随时换新液。
Mitochondrial staining with Heidenhain’s iron hematoxylin, both Chinese and foreign technical books have introduced, known as superior. But not as the book said, every dyed, and the narrative is not detailed enough, in order to better use the law in the future work, especially for beginners easy to grasp, we made a number of tests, come to some immature Experience and harvest. Now the actual operation under the column. 1. Drawn and fixed Take a young and lively mice, knocking the marrow by hand to death, immediately take the small intestine, pancreas, kidney and other small pieces of tissue, each about 2 mm long, quickly fixed to Regaud’s solution, the fixed The solution is slightly different from the original: 3% potassium dichromate 30ml (formerly 40ml) neutral Fmalin (calcium carbonate neutralization) 10ml fixed 4 days, followed by 3% potassium dichromate chromate for 8 days, all in the refrigerator Operation, daily renewal fluid, and found that the fluid black, at any time for new liquid.