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目的 探索大肠腺癌组织多药耐药基因 (MDR1)表达与大肠腺癌分化程度及肠系膜淋巴结转移之间的关系。方法 以光敏生物素标记MDR1cDNA探针 ,链亲和素胶体金为检测系统做原位杂交 (ISH SAG)检测大肠癌MDR1mRNA表达 ,同时用LSAB法对P 糖蛋白进行免疫组织化学检测。结果 大肠腺癌MDR1mRNA表达阳性的肠系膜淋巴结转移率与MDR1表达阴性的肠系膜淋巴结转移率两者有显著性差异 (P <0 0 5) ,大肠癌MDR1表达阳性率与分化程度无显著性差异(P >0 0 5)。结论 MDR1表达高低与大肠腺癌肠系膜淋巴结转移呈正相关 ,MDR1阳性表达与细胞分化程度比较无统计学意义。MDR1表达的检测对判定化疗疗效及预后有一定的意义。
Objective To explore the relationship between multidrug resistance gene (MDR1) expression in colorectal adenocarcinoma and the differentiation of colorectal adenocarcinoma and mesenteric lymph node metastasis. Methods The expression of MDR1 mRNA in colorectal cancer was detected by in situ hybridization (ISH SAG) with biotin labeled MDR1 cDNA probe and streptavidin colloidal gold as the detection system. The P glycoprotein was detected by immunohistochemistry with LSAB method. Results The rates of mesenteric lymph node metastasis of MDR1 mRNA in colorectal adenocarcinoma were significantly different from that of mesenteric lymph node metastasis in MDR1 (P <0.05) (P <0.05), while the positive rate of MDR1 in colorectal carcinoma was no significant difference (P > 0 0 5). Conclusion The expression of MDR1 is positively correlated with the mesenteric lymph node metastasis in colorectal adenocarcinoma. The expression of MDR1 is not related to the degree of cell differentiation. The detection of MDR1 expression has certain significance in judging the curative effect and prognosis of chemotherapy.