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目的建立HPLC法测定十味降糖胶囊中葛根素含量测定的方法。方法采用RP-HPLC法测定。用AgilentXDB C18色谱柱(250mm×4.6mm,5μm),以甲醇-磷酸盐溶液(20∶80)为流动相,流速为1mL.min-1,检测波长250nm,柱温为室温,进样量为10μL。结果葛根素保留时间约为27min左右,与其他峰的分离度大于2.0。葛根素的线性范围为0.024~0.476μg,r=0.9999,平均加样回收率为98.81%(n=5)。结论该方法简便快速,结果准确可靠,可用于十味降糖胶囊中葛根素的高效液相色谱法含量测定。
Objective To establish a HPLC method for the determination of puerarin in Shiwei Jiangtang capsules. The method was determined by RP-HPLC. Agilent XDB C18 column (250mm×4.6mm, 5μm) with methanol-phosphate solution (20:80) as mobile phase, flow rate 1mL.min-1, detection wavelength at 250nm, column temperature at room temperature, injection volume 10μL. Results The puerarin retention time was about 27min, and the resolution with other peaks was greater than 2.0. The linear range of puerarin was 0.024-0.476 μg, r=0.9999, and the average recovery was 98.81% (n=5). Conclusion The method is simple and rapid, and the result is accurate and reliable. It can be used for the determination of puerarin in Shiwei Jiangtang capsules by high performance liquid chromatography.