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目的:建立测定黄连解毒汤及其有效组分群中栀子苷、药根碱、黄芩苷、巴马汀和小檗碱含量的高效液相色谱方法。方法:采用Agilent TC-C18色谱柱(4.6mm×250mm,5μm),流动相为乙腈(A)-0.2%磷酸水(B);线性梯度洗脱程序:0~10min,10%~22%A;10~40min,22%~23%A;40~45min,23%~24%A;45~60min,24%A;检测波长:260nm;体积流量:1.0mL·min~(-1);进样量:10μL;柱温:25℃。结果及结论:该方法简单、可靠,其精密度、准确度、线性均符合要求,可用于黄连解毒汤及其有效组分群中有效成分的含量测定。
OBJECTIVE: To establish a HPLC method for determination of geniposide, jatrorrhizine, baicalin, palmatine and berberine in Huanglian Jiedu Decoction and its effective components. Methods: Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) was used with the mobile phase consisted of acetonitrile (A) -0.2% phosphoric acid water (B); linear gradient elution program: 0-10 min, 10-22% A ; 40 ~ 45 min, 23% ~ 24% A; 45 ~ 60 min, 24% A; detection wavelength: 260 nm; volume flow rate: 1.0 mL · min -1; Sample volume: 10 μL; column temperature: 25 ° C. RESULTS AND CONCLUSION: The method is simple and reliable, and its precision, accuracy and linearity meet the requirements. It can be used for the determination of active ingredients in Huanglian Jiedu Decoction and its effective components.