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首次应用培养人胎肺成纤维细胞 (FBs)损伤模型 ,体外研究创缘FBs合成细胞型纤维连接蛋白 (cellu larfibronectins,cFn)的变化与损伤时间的关系。结果表明 ,损伤使处于静息状态的融合FBs成为具有运动能力和增殖能力的活跃细胞。应用免疫组化ABC法结合图像分析技术 ,观察伤后不同时间创缘FBs内cFn的含量变化。伤后 1h ,可以检测到cFn有变化 ,且在伤后 6h内 ,cFn逐渐增多 ,其变化与损伤时间呈正相关。这对今后应用cFn与损伤时间推断的研究提供了科学依据 ,同时为法医学损伤时间研究开辟了新的途径。
The human fetal lung fibroblasts (FBs) injury model was firstly used to study the relationship between the changes of cFn and the injury time of FBs in vitro. The results showed that the injury made the resting FBs become active cells with motor ability and proliferation ability. The immunohistochemical ABC method combined with image analysis technique was used to observe the changes of cFn content in wound edge FBs at different time points. At 1 hour after injury, changes of cFn could be detected. Within 6 hours after injury, cFn gradually increased, and its change was positively correlated with injury time. This provides a scientific basis for the future application of cFn and injury time inference, and opens up new avenues for the study of forensic injury time.