栽培番茄品种“矮黄”的子叶作为外植体诱导愈伤组织 ,用 Na Cl进行直接高盐胁迫筛选。结果表明 ,2 2 5 mmol/LNa Cl直接高盐胁迫获得了耐盐突变体 ,1 0株完整的再生耐盐植株 ,在 1 5 0 mmol/L Na Cl的盐胁迫下 ,成活率可达 70 % ,而未经胁迫筛选过的原始株成活率则为零。其中 ,1株耐盐突变株能正常开花、结果。对其后代离体培养的茎尖和下胚轴外植体耐盐性进行比较 ,表明其后代仍然保持耐盐性 Cotyledons of cultivated tomato variety “Dwarf Yellow” were used as explants to induce callus and screened with NaCl directly. The results showed that salt tolerant mutants and 10 intact regenerated salt-tolerant plants were obtained under the direct salt stress of 250 mmol / L NaCl, and the survival rate was up to 70% under NaCl stress of 150 mmol / L NaCl %, While the surviving rate of the untreated original strain was zero. Among them, a salt tolerant mutant can flowering normally, the result. The salt tolerance of shoot explants and hypocotyl explants cultured in vitro of their offspring was compared, indicating that their offspring still maintained salt tolerance