论文部分内容阅读
目的:研究丹参酮IIA对3T3-L1脂肪细胞中脂联素表达和蛋白多聚化的作用。方法:用油红染色鉴定3T3-L1脂肪细胞的分化状态。用Real-time PCR检测基因的mRNA表达水平。用2%15%梯度SDS-PAGE及Western blot检测脂联素三种聚体。用siRNA技术进行基因沉默。结果:本研究发现传统中药丹参中的萘醌二萜类成分丹参酮IIA在脂肪细胞中激活AMPK通路,同时抑制脂肪细胞分化,降低脂联素的表达。而在前体脂肪细胞分化过程或成熟脂肪细胞中加入丹参酮IIA进行处理,均明显促进脂联素的多聚化,增加高聚体的浓度。抑制AMPK通路能够解除丹参酮IIA对脂联素的作用。结论:丹参酮IIA通过激活AMPK在3T3-L1脂肪细胞中促进脂联素的组装。
AIM: To investigate the effect of tanshinone IIA on adiponectin expression and protein multimerization in 3T3-L1 adipocytes. Methods: Oil red staining was used to identify the differentiation status of 3T3-L1 adipocytes. Real-time PCR was used to detect the gene mRNA expression level. Adiponectin triadimers were detected by 2% 15% gradient SDS-PAGE and Western blot. Gene silencing with siRNA technology. Results: The present study found that the tanshinone IIA, a naphthoquinone diterpenoid in the traditional Chinese medicine Salvia miltiorrhiza, activates AMPK pathway in adipocytes, inhibits adipocyte differentiation and decreases adiponectin expression. However, when Tanshinone IIA was added to the differentiation process of precursor adipocytes or mature adipocytes, adiponectin could be significantly increased and the concentration of polymer increased. Inhibition of AMPK pathway can relieve the effect of tanshinone IIA on adiponectin. Conclusion: Tanshinone IIA can promote adiponectin assembly in 3T3-L1 adipocytes by activating AMPK.