用放射性碘标记的多肽激素已广泛用于放射免疫测定和受体的研究。理想的标记物应与天然未标记物有完全相同的生物活性或免疫反应性胰岛素的碘化反应会产生不单一的碘化胰岛素的混和物。Freychet首先报道单碘胰岛素与未标记胰岛素有相同的生物活性。以后许多作者也成功地制备了单碘胰岛素,但这些标记物的碘化反应还不是定位的,而是胰岛素分子不同链和同一链上不同酪氨酸残基的单碘化,这种不同的单碘胰 Radioactive iodine labeled polypeptide hormones have been widely used in radioimmunoassay and receptor studies. The ideal label should have exactly the same biological activity as the native unlabeled or the iodinated reaction of the immunoreactive insulin will produce a mixture of not only iodinated insulin. Freychet first reported that mono-iodo-insulin has the same biological activity as unlabeled insulin. Many authors later succeeded in the preparation of mono-iodo-insulin, but the iodination of these labels is not yet localized, but mono-iodination of different tyrosine residues on different chains of the insulin molecule and on the same chain Single-iodine pancreas