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运用地高辛自由引物法标记1020bp CB cDNA 探针行斑点印迹杂交,检测胃癌手术标本中CBmRNA 含量。结果表明:胃癌组织中 CBmRNA 含量非常显著地高于非癌组织(P<0.01。提示 CB 在胃癌组织中的表达增高可能与胃癌的进展有关。
Digoxin-free primer method was used to mark the 1020bp CB cDNA probe for dot blot hybridization to detect the CBmRNA content in gastric cancer surgical specimens. The results showed that the content of CB mRNA in gastric cancer tissues was significantly higher than that in non-cancer tissues (P<0.01), suggesting that the increased expression of CB in gastric cancer tissues may be related to the progress of gastric cancer.