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宁夏枸杞(Lycium barbarum L.)叶外植体来源的愈伤组织经筛选、繁殖后,将来源相同、状态较为一致的淡黄色愈伤组织转移至O型或E型培养基上,可以诱导出器官发生和体细胞胚胎发生。利用该体系,对两条离体再生途径进行了比较研究。结果表明:(1)在拟分生组织和胚性细胞形成之前,RNA合成首先被激活,随后DNA、蛋白质合成加速;而球形胚形成期间,先是DNA合成的加快,接着RNA、蛋白质的合成高峰出现,在不定芽形成期间却正好相反;(2)可溶性蛋白组分发生规律性变化;器官发生和体细胞胚胎发生的启动阶段都有-153.6kD多肽出现,一些多肽分子在分化早期逐渐消失,而随芽原基或球形胚的形成又重新合成;与形态发生相对应,两种再生体系都有作为各自分子标记的特异多肽(84.9kD、46.3kD和44kD、36.2kD)的表达。此外,还对两种离体再生体系之间的关系和发生机制进行了讨论。
Lycium barbarum (Lycium barbarum L.) leaf explants derived from callus after screening, breeding, the same source, the same state of the light yellow callus transferred to O or E-type medium, can induce organogenesis And somatic embryogenesis. Using this system, two in vitro regeneration pathways were compared. The results showed that: (1) Before the meristems and embryogenic cells were formed, RNA synthesis was activated first, followed by accelerated DNA and protein synthesis. During the formation of globular embryos, DNA synthesis was accelerated first, followed by peak RNA and protein synthesis Appeared during the formation of adventitious buds, but the opposite occurred during the formation of adventitious buds; (2) the regular changes of soluble protein components occurred; both the initiation of organogenesis and somatic embryogenesis had -153.6kD peptides, some of the polypeptide molecules gradually disappeared in the early differentiation, But with the formation of bud primordium or globular embryos, they were synthesized again. Corresponding to the morphogenesis, the expression of specific molecular markers (84.9kD, 46.3kD and 44kD, 36.2kD) as their molecular markers in both regeneration systems. In addition, the relationship and mechanism of the two regeneration systems were discussed.