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[Objective] To compare the contents of volatile oil and germacrone in RHIZOMA CURCUMAE from different producing area such as Lingshan,Guiping and Guigang in Guangxi Province.[Method] The volatile oil was extracted from RHIZOMA CURCUMAE by steam distillation.The content of germacrone was determined by high performance liquid chromatography method (HPLC),and its chromatograph conditions were as follows:acetonitrile-water (V/V,70:30),flow rate of 1 ml/min,column temperature of 35 ℃,detection wavelength of 215 nm and sampling volume of 10 μl.[Result] The germacrone presented a good linear relation between the detection concentration of 0.022 3 and 0.139 8 mg/ml.Contents of volatile oil and germacrone in RHIZOMA CURCUMAE were varied for different producing area.[Conclusion] The contents of volatile oil and germacrone in RHIZOMA CURCUMAE from Lingshan were higher than that in other 8 areas.
[Objective] To compare the contents of volatile oil and germacrone in RHIZOMA CURCUMAE from different producing area such as Lingshan, Guiping and Guigang in Guangxi Province. [Method] The volatile oil was extracted from RHIZOMA CURCUMAE by steam distillation. The content of germacrone was determined by high performance liquid chromatography method (HPLC), and its chromatograph conditions were as follows: acetonitrile-water (V / V, 70:30), flow rate of 1 ml / min, nm and sampling volume of 10 μl. [Result] The germacrone presented a good linear relation between the detection concentration of 0.022 3 and 0.139 8 mg / ml. Contents of volatile oil and germacrone in RHIZOMA CURCUMAE were varied for different producing area. ] The contents of volatile oil and germacrone in RHIZOMA CURCUMAE from Lingshan were higher than that in other 8 areas.