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本实验是把显示葡萄糖-6-磷酸酶(G-6-Pase)的电镜细胞化学技术应用于研究急性砷中毒大鼠心肌G-6-Pase活性的变化,目的在于从亚显微水平上进一步探讨砷对心肌结构和功能的影响。选用健康wistar大鼠20只,随机分成实验组和对照组,每组10只。实验组大鼠每天自由饮用含As_2O_350PPm的蒸馏水,对照组饮用蒸馏水。35天后急性处死取其心尖部组织,置于4％多聚甲醛+1％戊二醛内前固定,入孵育液,37℃下孵育60min,1％锇酸后固定,常规脱水包埋,超薄切片,不经电子染色进行电镜观察。
In this study, we used the electron microscopy cytochemistry technique to display G-6-Pase in the study of myocardial G-6-Pase activity in acute arsenic poisoning rats. To investigate the effect of arsenic on myocardial structure and function. Twenty healthy wistar rats were randomly divided into experimental group and control group, with 10 rats in each group. Rats in the experimental group were allowed to drink distilled water containing As_2O_350PPm daily, while those in the control group were given distilled water. After 35 days, the apical tissues were sacrificed and fixed in 4% paraformaldehyde and 1% glutaraldehyde. The cells were incubated at 37 ° C for 60 min, fixed with 1% osmium tetroxide and routinely dehydrated and embedded Thin sections were electron micrographs without electron staining.