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目的:禾谷炭疽菌侵染玉米、小麦等农作物引起的炭疽病,给各国农业生产造成了巨大经济损失。植物病原菌在对植物侵染、定殖、扩展等致病过程中,细胞信号转导起着重要的作用。磷脂酰肌醇转移蛋白(PITP)参与众多生理生化过程,并在细胞信号转导过程中发挥重要作用。本研究为深入开展禾谷炭疽菌PITP的功能研究打下坚实的理论基础,同时,也为进一步开展其他炭疽菌的研究提供重要的理论指导。方法:基于酿酒酵母中已经报道的5个典型PITP序列,对炭疽菌属蛋白质数据库进行Blastp比对以及关键词搜索,并通过SMART保守结构域分析。结果:明确禾谷炭疽菌存在3个典型的PITP,同时,明确上述蛋白疏水性、细胞信号肽、跨膜区结构、亚细胞定位以及二级结构等情况。结论:与其他物种中9个同源序列进行遗传关系比较分析,该菌与希金斯炭疽菌亲缘关系较近。
Objective: Anthracnose infestations of anthracnose caused by crops such as corn and wheat have caused great economic losses to agricultural production in various countries. Plant pathogens in plant infection, colonization, expansion and other pathogenic processes, cell signaling plays an important role. Phosphatidylinositol transfer protein (PITP) is involved in many physiological and biochemical processes and plays an important role in cellular signal transduction. This study lays a solid theoretical foundation for the further study on the function of PITP in M. cerealis and provides important theoretical guidance for further research on other anthrax species. Methods: Based on the five typical PITP sequences reported in Saccharomyces cerevisiae, the Blastp alignment and keyword search of the anthracnose protein database were performed and analyzed by the SMART conserved domain. Results: Three typical PITPs were identified in M. cereus. At the same time, the protein hydrophobicity, cell signal peptide, transmembrane structure, subcellular localization and secondary structure were all clearly identified. Conclusion: The genetic relationships among 9 homologous sequences of other species are comparatively analyzed. The genetic relationship between this strain and Higgins anthracnose is relatively close.