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目的:探索99Tcm-4,9-二氮-3,3,10,10-四甲基十二烷-2,11-二酮肟(HL91)在局部脑缺血模型脑组织中分布特点以及99Tcm-HL91检测急性脑卒中缺血半暗带的可能性。方法:45只SD大鼠,其中有14只用于研究99Tcm-HL91在正常大鼠体内的生物分布情况,有15只用于研究99Tcm-HL91在局部脑缺血模型的脑组织中分布特点,有16只动物模型进行了脑组织放射性自显影研究。结果:肝脏和肾脏的99Tcm-HL91摄取比其他组织高,注射后1h,小肠的摄取也比较高,但脑组织中的放射性较低。缺血部位(靶区)的放射性摄取较对侧相应部位高,手术各亚组和假手术各亚组间的差异具有统计学意义(t=2.9～23.11,P<0.05～0.001)。动物模型脑组织自显影图像可见靶区有局限性的99Tcm-HL91选择性摄取,经计算机图像分析系统进行图像分析和统计学处理后,同一时相靶区与非靶区的单位面积净吸光度差异有显著性(t=4.423～12.277,P<0.05),各时相靶/非靶比值与对照比较差异均有显著性(t=3.73～12.39,P<0.01),且随着注射药物的时间延长而增高。结论:99Tcm-HL91能够选择性地浓集于缺血缺氧的脑组织中,清除延缓,可用于急性脑卒中缺血半暗带的探测。
Objective: To investigate the distribution of 99Tcm-4,9-diazonia-3,10,10-tetramethyldodecane-2,11-dione oxime (HL91) in brain tissue of focal cerebral ischemia model -HL91 detects the possibility of ischemic penumbra in acute stroke. METHODS: Forty-five Sprague-Dawley rats were used to study the biodistribution of 99Tcm-HL91 in normal rats. Fifteen of them were used to study the distribution of 99Tcm-HL91 in brain tissue of rats with focal cerebral ischemia. Twenty-six animal models were studied for brain autoradiography. Results: The uptake of 99Tcm-HL91 in liver and kidney was higher than that of other tissues, and the ingestion of small intestine was also higher at 1 hour after injection, but the radioactivity in brain tissue was lower. The radioactive uptake of the ischemic site (target area) was higher than that of the corresponding contralateral side. The differences between the subgroups and the subgroups of sham operation were statistically significant (t = 2.9-23.11, P <0.05-0.001). The animal model of brain tissue self-developed images showed selective target 99Tcm-HL91 selective uptake, image analysis and statistical analysis by the computer image analysis system, the same time the target area and non-target area net absorbance difference per unit area (T = 4.423 ~ 12.277, P <0.05). The target / non-target ratio at each time point was significantly different from the control (t = 3.73-12.39, P <0.01) Extend and increase. CONCLUSION: 99Tcm-HL91 can be selectively concentrated in the brain tissue of hypoxia and hypoxia, and its debridement is delayed. It can be used to detect the ischemic penumbra in acute stroke.