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目的观察颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响。方法雄性成年大鼠40只随机分为3组:对照组(n=10,腹腔注射注射等量缓冲液),糖尿病组(n=15,采用STZ糖尿病模型按65 mg/kg剂量腹腔1次注射1%STZ制备糖尿病大鼠),糖尿病+颈交感神经阻滞组(n=15,按65 mg/kg剂量腹腔1次注射1%STZ,糖尿病成模后8周给予颈交感神经阻滞治疗)。造模后12周水迷宫实验观察各组大鼠的认知功能。并于12周处死大鼠,采用Clark氧电极法测定大鼠海马线粒体的呼吸功能,免疫组化检测海马IL-6、TNF-α的表达。结果造模后12周,糖尿病组存活12只,阻滞组存活13只。糖尿病组、阻滞组与对照组相比,逃避潜伏期明显延长、穿越平台的次数明显减少(P<0.05)。糖尿病组线粒体呼吸功能为R3(28.51±3.01)、R4(13.93±1.16)、RCR(2.09±0.27),阻滞组的为R3(45.26±4.21)、R4(11.99±1.11)、RCR(3.52±0.32),对照组为R3(66.31±5.65)、R4(12.97±3.38)、RCR(4.78±0.68),糖尿病组和阻滞组线粒体呼吸功能均较对照组组明显下降,主要表现为R3和RCR值显著降低(P<0.05);但阻滞组的R3和RCR高于糖尿病组(P<0.05)。在海马CA1区,糖尿病组IL-6、TNF-α表达为(53.32±6.29)、(63.21±13.24),阻滞组为(28.14±3.82)、(32.76±5.98),均高于对照组(13.87±2.54)、(11.09±8.87)(P<0.05)。阻滞组明显低于糖尿病组,差异有统计学意义(P<0.05)。结论颈交感神经阻滞可改善糖尿病大鼠脑线粒体功能,其机制可能与其下调IL-6、TNF-α有关。
Objective To observe the effects of cervical sympathetic nerve block on mitochondrial function in diabetic rats. Methods Forty male adult rats were randomly divided into three groups: control group (n = 10), intraperitoneal injection of equal volume of buffer solution, diabetic group (n = 15), intraperitoneal injection of 65 mg / kg STZ diabetes model 1% STZ), diabetic + cervical sympathectomy group (n = 15, intraperitoneal injection of 1% STZ at a dose of 65 mg / kg and cervical sympathetic nerve block treatment at 8 weeks after diabetes mellitus) . After 12 weeks of modeling, the water maze test was used to observe the cognitive function of rats in each group. The rats were sacrificed at 12 weeks. The respiratory function of rat hippocampal mitochondria was measured by Clark oxygen electrode method. The expressions of IL-6 and TNF-α were detected by immunohistochemistry. Results Twelve weeks after modeling, 12 patients in the diabetic group and 13 in the blocking group survived. Compared with the control group, the escape latency and the number of crossing the platform in diabetic group and blocking group were significantly longer (P <0.05). The respiratory function of mitochondria in diabetic group was R3 (28.51 ± 3.01), R4 (13.93 ± 1.16), RCR (2.09 ± 0.27), R3 (45.26 ± 4.21), R4 0.32). In the control group, the mitochondrial respiration rate of R3 (66.31 ± 5.65), R4 (12.97 ± 3.38) and RCR (4.78 ± 0.68) were significantly lower in the diabetic group and the block group than in the control group (P <0.05). However, the R 3 and RCR values in the block group were significantly higher than those in the diabetic group (P <0.05). The expression of IL-6 and TNF-α in diabetic group was (53.32 ± 6.29) and (63.21 ± 13.24) in the diabetic group and (28.14 ± 3.82) and (32.76 ± 5.98) in the retardation group compared with the control group 13.87 ± 2.54), (11.09 ± 8.87) (P <0.05). The block group was significantly lower than the diabetic group, the difference was statistically significant (P <0.05). Conclusion Cervical sympathetic nerve block can improve the mitochondrial function in diabetic rats, which may be related to down-regulation of IL-6 and TNF-α.