以六倍体高加索三叶草Trifolium ambiguum与四倍体白三叶T.repens为亲本,通过远缘杂交,采用组织培养手段对杂交胚的萌发条件进行探索。结果表明:以高加索三叶草为母本、白三叶为父本,在花期8:00—9:00授粉,授粉后12~13 d取胚,用质量分数10%NaClO灭菌6.5~7 min,在不加任何外源激素的改良MS培养基上,胚萌发率最高,达8%。高频扩繁时,再附加0.25 mg/L 2,4-D+0.50 mg/L 6-BA可获得较高萌发率。 The germination conditions of hybrid embryos were explored by distant crosses and tissue culture using the hexaploid Caucasian Trifolium ambiguum and tetraploid T. clover as parents. The results showed that embryos were white clover and white clover were pollinated at 8: 00-9: 00 and pollinated 12-13 days after pollination. The embryos were sterilized with 10% NaClO for 6.5-7 min, On the modified MS medium without any exogenous hormones, germination rate was the highest at 8%. In the high-frequency propagation, higher germination rate could be obtained by adding 0.25 mg / L 2,4-D + 0.50 mg / L 6-BA.