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建立了液相色谱-串联质谱法同时测定青娥丸中的3种药效成分——松脂醇二葡萄糖苷、补骨脂素和异补骨脂素。采用Kromasil C18色谱柱,以甲醇-0.1%甲酸为流动相进行梯度洗脱,使用多反应监测(MRM)模式,监测离子对为m/z 700.5→m/z 235.4(松脂醇二葡萄糖苷)和m/z 187.1→m/z 131.1(补骨脂素和异补骨脂素)。3种成分的平均回收率为99.1%、99.0%和98.6%,RSD为1.33%、1.43%和1.27%。
A liquid chromatography-tandem mass spectrometry (LC-MS / MS) method was developed for the simultaneous determination of three medications, Pinoresinol diglucoside, Psoralen and Isopsoralen, in Qingyang pills. A Kromasil C18 column was used with gradient elution with methanol-0.1% formic acid as the mobile phase. The multi-reaction monitoring (MRM) mode was used and the ion pairs monitored were m / z 700.5 → m / z 235.4 (pinoresinol diglucoside) m / z 187.1 → m / z 131.1 (psoralen and isopsoralen). The average recoveries of the three components were 99.1%, 99.0% and 98.6% with RSDs of 1.33%, 1.43% and 1.27%.