【摘 要】
：
In order to investigate the inhibition effect of Tat protein, an HIV-1 encoding transactivator, on HIV-1 replication, M5 and M6 Tat retroviral vectors which ca
【机 构】
：
State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Acad
【出 处】
：
Progress in Natural Science
论文部分内容阅读
In order to investigate the inhibition effect of Tat protein, an HIV-1 encoding transactivator, on HIV-1 replication, M5 and M6 Tat retroviral vectors which can express the mutant Tat proteins, M5 (Arg52→Leu) and M6 (Thr40→Ala, Lys41→Ala, meanwhile premature at Lys50) respectively, were constructed and transfected into the amphotropic PA317 cells. Deduced replication-deficiency retrovirus further infected MT4 cells. HIV-1 challenge experiments showed that M5 and M6 Tat proteins could both inhibit the replication of HIV-1.
In order to investigate the inhibition effect of Tat protein, an HIV-1 encoding transactivator, on HIV-1 replication, M5 and M6 Tat retroviral vectors which can express the mutant Tat proteins, M5 (Arg52 → Leu) and M6 (Thr40 → Ala , Lys41 → Ala, meanwhile premature at Lys50) respectively, were constructed and transfected into the amphotropic PA317 cells. Deduced replication-deficiency retrovirus further infected MT4 cells. HIV-1 challenge experiments showed that M5 and M6 Tat proteins could both inhibit the replication of HIV-1.
其他文献
近年来,笔者以自拟复方速效止咳汤治疗咳嗽62例,取得较满意的速效止咳之效,现介绍如下。药物组成和治疗方法复方速效止咳汤由生侧柏叶10克,百部12克,桔梗9克,款冬花12克,紫
信用卡在不久的将来可能会被智能笔所取代。这种智能笔会捕捉使用者的指纹,并判断在票据上签字的人是否是自己真正的主人。用智能笔来取代信用卡,任何欺诈行为都将行不通,因
由于种种原因,我们对孕产妇的心理因素的重视程度还远远达不到发达国家的水平,尤其是对临产妇的心理隐患重视不够。因此作者根据近年来的观察,浅谈一下自己的看法。 临产妇
两头尖为毛莨科植物多被银莲花(Anemneraddeana Regel)的干燥根茎。明·刘文泰所著《本草品汇精要》谓两头尖:“味辛性热,有毒。疗风及腰腿疼痛。”近代《中药志》等书指出
ＯｂｊｅｃｔｉｖｅＴｏｄｅｖｅｌｏｐｔｈｅｖａｃｉｎｅｏｆＣｈｉｎｅｓｅＳｃｈｉｓｔｏｓｏｍｉａｓｉｓｊａｐｏｎｉｃｕｍ，ｗｅｔｒｙｔｏｐｒｅｐａｒｅｔｈｅ２３ｋＤａｍｅｍｂｒａｎｅｐｒｏｔｅｉｎｏｆＳｃｈｉｓｔｏｓｏｍａｊａｐｏｎｉｃｕｍＣｈｉｎｅ...
ObjectiveTodevelopmentvacineofChineseSchistosomiasisjaponicum, wetrytopreparethe23kDamembraneproteinofSchistosomajaponicumChi
２００３年是全面贯彻落实党的十六大精神的第一年，也是实施“十五”计划的关键一年。为适应新形势、新任务，进一步提高《江苏统计》的质量和影响力，本刊在今后的编辑工作中将与广大作者
自 1994年起，连续举办了七年的“中国国际包装工业展览会” (简称“包装工业展” )将于 2001年 3月 6～ 9日在中国广州市中国出口商品交易会展馆 (广交会会场 )举行第八届展会。
【十二日愈】语出10条。是约略之词,不能看成定论,表解一般在七日之间,更加七日,则正气渐复,邪气渐去,所以“十二日愈”。柯韵伯曰:“七日表解后,复过一候,而五脏元气始亢,
检测幽门螺杆菌（HelicobacterPylori,Hp）感染胃炎患者鲁窦粘膜体外培养上消液中肿瘤坏死因子（TumorNecrosisFactor－α，TNF－α）和血小板源生长因子（Platelet－derivedGrowthFactor，PDGF）水
[答] 血竭又称骐驎竭、血结。早在《唐本草》中就有记载。它是棕榈科(Palmae)植物麒麟竭calamus draco willdenow及同属其它植物的果实和树干渗出的一种树脂经加热蒸压而成