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目的采用HPLC-ELSD建立益气祛白颗粒中黄芪甲苷的含量测定方法。方法采用Agilent Eclipse XDB-C18ODS色谱柱(250 mm×4.6 mm,5μm);柱温30℃;乙腈-双蒸水溶液(32∶68)为流动相;流速1 mL·min-1;ELSD漂移管温度60℃;载气(氮气)流量1.5 L·min-1。结果黄芪甲苷含量测定的线性范围为0.025 2~0.631 g·L-1,线性方程为y=1.3499x+10.202,r=0.999 1(n=5),平均回收率为102.4%,RSD=2.0%(n=9)。结论 HPLC-ELSD法准确、可靠、重复性好,可用于益气祛白颗粒中黄芪甲苷的含量测定。
OBJECTIVE To establish a HPLC-ELSD method for the determination of Astragaloside IV in Yiqi Qubai granules. Methods The column temperature was 30 ℃ and acetonitrile-double distilled water (32:68) was used as the mobile phase. The flow rate was 1 mL · min-1. The temperature of ELSD drift tube 60 ℃; carrier gas (nitrogen) flow 1.5 L · min-1. Results The linear range was 0.025 2 ~ 0.631 g · L -1. The linear equation was y = 1.3499x + 10.202, r = 0.999 1 (n = 5). The average recoveries were 102.4% and RSD = 2.0 % (n = 9). Conclusion The HPLC-ELSD method is accurate, reliable and reproducible. It can be used to determine the content of Astragaloside IV in Yiqi Jubai Granule.