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目的 探讨粘附分子P-选择素与树突状细胞(DC)在大鼠单侧输尿管梗阻后肾组织中的表达和分布,以及抗P-选择素单抗的抗粘附调抑作用。方法 建立大鼠单侧输尿管梗阻(UUO)模型,随机将大鼠分为手术组和抗P-选择素单抗治疗组各18只,按不同梗阻时间(1、3、7d)再分为3组,另设假手术组(n=6)作为对照。分别采用免疫组化LSAB法和免疫双染与荧光图像分析法,观察P-选择素及DC在肾组织中表达和分布变化。结果 大鼠UUO第1d起即见P-选择素以肾小管上皮细胞为主在肾小管间质中表达上调,伴随CD1a~+CD80~+DC在以肾间质为主部位的浸润;第3 d P-选择素明显表达,且CD1a~+CD80~+DC显著增多;至第7 d P-选择素表达遂下调,而CD1a~+CD80~+DC在肾间质分布聚集达峰,出现了明显的肾小管间质病理损伤经抗P-选择素单抗处理后,大鼠肾组织P-选择素表达受到抑制,CD1a~+CD80~+DC分布聚集减少,且肾小管间质病理损伤减轻。结论 提示树突状细胞参与了肾小管间质纤维化早期病理损伤过程,其肾组织迁移聚集可能与P-选择素早期介导有关,抗P-选择素单抗对此具有抗粘附调抑和肾脏保护作用。
Objective To investigate the expression and distribution of adhesion molecules P-selectin and dendritic cells (DCs) in renal tissues after unilateral ureteral obstruction in rats and the anti-adhesion inhibition effect of anti-P-selectin monoclonal antibody. Methods Unilateral ureteral obstruction (UUO) model was established in rats. The rats were randomly divided into two groups: operation group and anti-P-selectin monoclonal antibody treatment group, each group was divided into three groups according to different obstruction time (1,3,7d) Group, another sham operation group (n = 6) as a control. Immunohistochemical staining and immunohistochemical staining were used to detect the expression of P-selectin and DC in renal tissues. Results From the first day of rat UUO, P-selectin was mainly expressed in renal tubular epithelial cells in the tubulointerstitium, accompanied by the infiltration of CD1a ~ + CD80 ~ + DC in the interstitium-dominated sites. In the third d P-selectin was significantly increased, and CD1a ~ + CD80 ~ + DC significantly increased; by the seventh day the expression of P-selectin was down-regulated, while the distribution of CD1a ~ + CD80 ~ + DC in the renal interstitium peaked, Significant tubulointerstitial pathological changes After treatment with anti-P-selectin monoclonal antibody, the expression of P-selectin in rat renal tissue was inhibited, the distribution of CD1a ~ + CD80 ~ + DC was reduced and the pathological damage of tubulointerstitial lesion was alleviated . Conclusions Dendritic cells are involved in the process of early pathological injury of tubulointerstitial fibrosis. The migration and aggregation of renal tissue may be related to the early mediation of P-selectin. Anti-adhesion inhibition of anti-P-selectin monoclonal antibody And kidney protection.