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目的:探讨人卵巢上皮性癌组织中Pim-1和C-myc的表达及其临床意义。方法:采用RT-PCR半定量方法和免疫组织化学法检测40例卵巢上皮性癌组织、20例卵巢上皮性良性肿瘤组织和10例正常卵巢组织中Pim-1和C-myc mRNA及蛋白的表达情况。结果:卵巢上皮性癌组织中Pim-1和C-myc mRNA与蛋白的表达水平明显高于卵巢上皮性良性肿瘤组织及正常卵巢组织(P<0.05);Pim-1蛋白在卵巢上皮性癌组织中的表达与其组织学类型无关(P>0.05),与国际妇产科联盟(Federation International of Gynecology and Obstetrics,FIGO)分期和淋巴结转移有关(P<0.05);C-myc蛋白在卵巢上皮性癌组织中的表达与其组织学类型、FIGO分期和淋巴结转移均无关(P>0.05)。结论:原癌基因Pim-1和C-myc的mRNA及蛋白在卵巢上皮性癌组织中过度表达,可能与卵巢上皮性癌的发生、发展有关。
Objective: To investigate the expression of Pim-1 and C-myc in human ovarian epithelial carcinoma and its clinical significance. Methods: The mRNA and protein expressions of Pim-1 and C-myc in 40 cases of epithelial ovarian cancer, 20 cases of benign ovarian tumor and 10 cases of normal ovarian tissue were detected by semi-quantitative RT-PCR and immunohistochemistry Happening. Results: The mRNA and protein expressions of Pim-1 and C-myc in epithelial ovarian cancer tissues were significantly higher than those in benign epithelial ovarian tumors and normal ovarian tissues (P <0.05). The expression of Pim-1 protein in ovarian epithelial carcinoma (P <0.05). The expression of C-myc in epithelial ovarian cancer was significantly higher than that in the histological type (P> 0.05) Tissue expression was not associated with histological type, FIGO stage and lymph node metastasis (P> 0.05). Conclusion: The overexpression of Pim-1 and C-myc mRNA and protein in epithelial ovarian cancer may be related to the occurrence and development of epithelial ovarian cancer.