对灰树花深层发酵菌丝体多糖的酶法提取工艺及其抗肿瘤作用进行了研究 .结果表明 :利用复合酶法处理并结合热水浸提 ,缩短了提取时间 ,且能显著提高多糖的提取率 ,多糖提取率达7.4 % ,效果优于其他浸提方法 .动物体内实验证明 ,腹腔注射菌丝体多糖对小鼠移植性肿瘤S180有明显的抑制作用 ,抑制率达 57.6 %～ 6 6 .7% . The enzymatic extraction and anti-tumor effects of polysaccharides from the deep fermentation mycelia of Grifola frondosa were studied. The results showed that the use of complex enzymatic treatment combined with hot water extraction shortened the extraction time and significantly improved the polysaccharides The extraction rate, polysaccharide extraction rate of 7.4%, the effect is better than other extraction methods. Animal experiments in vivo showed that intraperitoneal injection of mycelial polysaccharides on the transplanted tumor S180 significantly inhibited the inhibitory rate of 57.6 % to 6 6 .7% .