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本文提出了应用薄层层析分离和比色测定注射液中维生素D_2及D_3含量的方法。油注射液中维生素D_2的测定:吸取麦角骨化醇标准溶液[1毫克/毫升的氯仿与向日葵油(10∶1)混合液]相当于维生素D_2 3.00、5.00、7.00微克及样品溶液(1.5%注射液5毫升以氯仿稀释至50毫升)相当于3.00微克,点滴于硅胶薄层上,以氯仿为展开剂进行层析至前沿达10厘米,展开后取出薄板置于空气中干燥5分钟,用饱和(22%)三氯化锑的氯仿溶液喷雾显色,几分钟后以光密度计进行定量测定。以标准显出斑点的Δ1%/10g C绘制校正曲线,从校正曲线中求得样品的含量。
This paper presents a method for the determination of vitamin D 2 and D 3 in injection by TLC and colorimetric methods. Determination of vitamin D 2 in oil injection: The ergocalciferol standard solution [10 mg / ml in chloroform and sunflower oil (10: 1)] is equivalent to 3.00, 5.00 and 7.00 μg vitamin D 2 and 1.5% Injection of 5 ml chloroform diluted to 50 ml) equivalent to 3.00 micrograms, drip on a thin layer of silica gel, chloroform as the developing agent to the front of the chromatography up to 10 cm, after unwinding the sheet was left in the air and dried for 5 minutes with A saturated (22%) antimony trichloride solution in chloroform was spray-developed and a few minutes later quantified by densitometry. Draw a calibration curve at Δ1% / 10g C of the standard spot, and determine the sample content from the calibration curve.