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目的研究白细胞介素10(IL-10)基因启动子区-592A>C单核苷酸多态性(SNP)与广东汉族妇女宫颈癌易感的关系。方法利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析技术,对70例宫颈癌患者和108名健康对照个体的IL-10-592A>C多态位点进行分型,比较各组间等位基因及基因型频率的分布,分析该多态位点与宫颈癌易感的相关性。结果AA、AC、CC基因型在病例组中的频率分别为50.0%、32.9%和17.1%,在对照组中分别是47.3%、40.7%和12.0%,两组间基因型频率分布差异无统计学意义(χ2=1.56,P=0.459);以AA基因型作为参照,AC和CC基因型均没有显著增加个体患宫颈癌的风险(OR=0.76,95%C I=0.39-1.48,P=0.421以及OR=1.35,95%C I=0.55-3.29,P=0.516)。结论IL-10基因基因启动子区-592A>C多态位点与广东汉族妇女人群宫颈癌的易感性无关。
Objective To investigate the relationship between -592A> C SNP in promoter region of interleukin 10 (IL-10) gene and susceptibility to cervical cancer in Guangdong Han women. Methods Polymorphisms of IL-10-592A> C polymorphisms in 70 cervical cancer patients and 108 healthy individuals were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) The distribution of alleles and genotype frequencies was compared between groups, and the association between polymorphism sites and susceptibility to cervical cancer was analyzed. Results The frequencies of AA, AC and CC genotypes were 50.0%, 32.9% and 17.1% in the case group and 47.3%, 40.7% and 12.0% in the control group respectively. There was no statistical difference in genotype distribution between the two groups (Χ2 = 1.56, P = 0.459). There was no significant difference in AC and CC genotypes between the two groups (OR = 0.76, 95% CI = 0.39-1.48, P = 0.421 And OR = 1.35, 95% CI = 0.55-3.29, P = 0.516). Conclusion The -592A> C polymorphism in IL-10 gene promoter region is not associated with the susceptibility of cervical cancer in Han women of Guangdong.