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目的研究胰岛素样生长因子-1(IGF-1)在非梗阻性无精子症睾丸组织中的表达并探讨其意义。方法采用免疫组化EnVision法对32例非梗阻性无精子症人睾丸组织石蜡标本和13例人正常睾丸组织中的IGF- 1的表达进行检测,通过统计学方法分析其表达情况。结果非梗阻性无精子症睾丸组织HE染色显示生精小管中无或仅少量精子,生精小管的生精上皮脱落、排列紊乱,生精小管壁部分有玻变萎缩。32例非梗阻性无精子症睾丸组织中IGF-1阳性表达4例,阴性表达28例,阳性率12.5%;13例正常睾丸组织中IGF-1阳性表达10例,阴性表达3例,阳性率76.9%;差异有显著性(P<0.01)。结论(1)IGF-1在睾丸组织中表达减少或缺失与生精功能障碍密切相关。(2)非梗阻性无精子症睾丸组织生精小管的生精上皮脱落、排列紊乱,生精阻滞。
Objective To study the expression of insulin-like growth factor-1 (IGF-1) in the non-obstructive azoospermia testis and its significance. Methods Immunohistochemical EnVision method was used to detect the expression of IGF-1 in 32 cases of non-obstructive azoospermia testis tissue specimens and 13 cases of normal human testis tissue, and its expression was analyzed by statistical methods. Results Non-obstructive azoospermia testis HE staining showed no or only a small amount of spermatogenesis in seminiferous tubules, seminiferous tubules of the seminiferous epithelial shedding, disorganized, part of the seminiferous tubule atrophy. In 32 cases of non-obstructive azoospermia testicular tissue, 4 cases were positive for IGF-1, 28 cases were negative, the positive rate was 12.5%. In 13 cases of normal testicular tissue IGF-1 was positive in 10 cases and negative in 3 cases 76.9%; the difference was significant (P <0.01). Conclusion (1) The decrease or loss of IGF-1 in testis tissue is closely related to dysfunction of spermatogenesis. (2) non-obstructive azoospermia testicular seminiferous tubules of spermatogenic epithelial shedding, disordered, spermatogenic arrest.