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心肌缺血45min，再灌注180min复制兔急性心肌缺血再灌注模型。分别观察了等容血液稀释、维拉帕米以及二者联合应用对兔缺血再灌注心肌MDA含量、SOD活性及心功能的影响。实验并分四组：Ⅰ组（对照组）；Ⅱ组（稀释组）；Ⅲ组（维拉帕米组）；Ⅳ组（稀释＋维拉帕米组）。结果：①心肌组织MDA含量及SOD活性变化：与Ⅰ组缺血区心肌MDA含量相比，Ⅱ、Ⅲ、Ⅳ组均显著降低,Ⅳ组又明显低于Ⅱ、Ⅲ组；Ⅱ、Ⅲ、Ⅳ组缺血区心肌SOD活性均明显高于Ⅰ组（均P＜0．05）。②心功能变化：再灌注后，Ⅰ组心功能进行性恶化，Ⅱ组左室收缩功能（LVSP，＋dp／dt－max）和舒张功能（-dp／dt－max）有明显改善，Ⅲ组舒张功能明显改善，Ⅳ组各项心功能指标进一步好于Ⅱ、Ⅲ组（均P＜0．05）。提示：等容血液稀释和维拉帕米均能保护缺血再灌注心肌的代谢和功能，二者合用效果更加明显．表现出良好的协同效应。
Myocardial ischemia 45min, 180min after reperfusion rabbit model of acute myocardial ischemia-reperfusion. The effects of isovolemic hemodilution, verapamil and their combination on MDA content, SOD activity and cardiac function in ischemia-reperfusion myocardium were observed. The experiment was divided into four groups: group Ⅰ (control group); group Ⅱ (dilution group); group Ⅲ (verapamil group); group Ⅳ (dilution + verapamil group). Results: ① The changes of MDA content and SOD activity in myocardium: Compared with those in group Ⅰ, the levels of MDA in group Ⅱ, Ⅲ and Ⅳ were significantly lower than those in group Ⅱ, while those in group Ⅳ were significantly lower than those in groups Ⅱ and Ⅲ The activity of SOD in ischemia group was significantly higher than that in group Ⅰ (all P <0.05). Cardiac function changes: After reperfusion, the cardiac function in group Ⅰ was deteriorated. The LVSP, dp / dt-max and diastolic function (-dp / dt-max) were significantly improved in group Ⅱ, Function improved significantly, each group Ⅳ heart function index is better than Ⅱ, Ⅲ group (all P <0.05). Hint: Isotonic hemodilution and verapamil both protect the metabolism and function of ischemic-reperfused myocardium, and the combined effect is more obvious. Show a good synergistic effect.