论文部分内容阅读
目的探讨运动训练前吸入不同量的氧气对小鼠运动耐力的影响。方法昆明种小鼠按游泳时间随机分为无运动训练不吸氧组、运动训练不吸氧组、运动训练前吸氧30 min组、运动训练前吸氧60 min组、运动训练前吸氧90min组,每组各30只,吸入氧气浓度为47%,运动训练时间为30 min。20 d后,每组取15只取血,其余15只测定游泳力竭时间,然后取血测定血清乳酸、SOD和MDA。结果运动训练前吸氧30 min组的力竭时间明显延长(P<0.001,P=0.041,P=0.011,P<0.001);安静状态下,运动训练前吸氧30 min组的乳酸值明显降低(P=0.002,P=0.009,P=0.022,P=0.011);经过运动训练后的各组SOD活力均高于非运动训练组(P<0.001,P<0.001,P<0.001,P<0.001);运动训练前吸氧30 min组与其他2个吸氧组相比MDA含量较低(P=0.003,P=0.002)。力竭后,运动训练前吸氧30 min组的乳酸与其他组相比差异有统计学意义(P<0.001,P=0.001,P=0.001,P<0.001);运动训练前吸氧30 min组与其他2个吸氧组相比SOD活力较低(P<0.001,P<0.001)、MDA含量也较低(P=0.014,P<0.001)。结论运动训练前吸入适量的氧气可以延缓小鼠的疲劳,加快体内自由基的清除,增强运动能力。相反,吸入大量氧气可能会使自由基紊乱,减少运动耐力。
Objective To investigate the effect of different levels of oxygen inhalation on exercise tolerance in mice before exercise training. Methods Kunming mice were divided randomly into swimming no-oxygen training group, exercise-training non-oxygen-absorbing group, 30 min before exercise training, 60 min before exercise training and 90 min before exercise training Groups, 30 in each group, inhaled oxygen concentration of 47%, exercise training time was 30 min. After 20 days, 15 blood samples were taken from each group, and the remaining 15 rats were subjected to exhaustive swimming test. Serum lactate, SOD and MDA were determined by blood sampling. Results The exhaustion time of 30 min before exercise training was significantly prolonged (P <0.001, P = 0.041, P = 0.011, P <0.001). In quiet state, the lactic acid value of 30 min before exercise training was significantly decreased (P = 0.002, P = 0.009, P = 0.022, P = 0.011). The SOD activity in each group after exercise training was higher than that in non-exercise training group (P <0.001, P <0.001, P <0.001, P <0.001) ); Compared with the other two oxygen inhalation groups, the content of MDA in 30 min before exercise training was lower (P = 0.003, P = 0.002). After exhaustive exercise, there was a significant difference between the 30 min before exercise training and the other groups (P <0.001, P = 0.001, P = 0.001, P <0.001) Compared with the other two oxygen inhalation groups, SOD activity was lower (P <0.001, P <0.001) and MDA content was lower (P = 0.014, P <0.001). Conclusions Inhaling the right amount of oxygen before exercise training can delay the fatigue of mice, accelerate the clearance of free radicals and enhance the exercise capacity. Conversely, inhaling large amounts of oxygen may disrupt free radicals and reduce exercise tolerance.