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目的探讨尾型同源盒转录因子-2(CDX-2)和抑癌基因KAI-1在结肠癌中的表达并分析其临床意义。方法应用免疫组织化学SP法检测50例结肠癌组织及相应癌旁组织和25例正常结肠黏膜组织中CDX-2和KAI-1蛋白的表达,分析其表达与结肠癌患者临床病理特征的关系以及二者表达的相关性。结果①在结肠癌组织及相应癌旁组织(距癌组织≤2 cm)和正常结肠黏膜组织中CDX-2蛋白表达阳性率分别为34%(17/50)、54%(27/50)及88%(22/25),KAI-1蛋白表达阳性率分别为30%(15/50)、58%(29/50)及92%(23/25),CDX-2和KAI-1在结肠癌组织中的蛋白表达阳性率均分别明显低于相应癌旁组织(P<0.05)及正常结肠黏膜组织(P<0.05),其在癌旁组织中的表达阳性率也均明显低于正常结肠黏膜组织(P<0.05)。②CDX-2和KAI-1蛋白表达阳性率与结肠癌淋巴结转移、浸润深度及分化程度均有关(P<0.05),即在有淋巴结转移、浸润深度浸及浆膜及分化程度较低者中CDX-2和KAI-1蛋白表达阳性率均明显低于其在无淋巴结转移、浸润深度未及浆膜及分化程度较高者(P<0.05),二者均与患者的发病年龄和性别无关(P>0.05)。③Spearman等级相关分析表明,CDX-2和KAI-1蛋白阳性表达呈正相关(r s=0.544,P<0.01)。结论 CDX-2和KAI-1的表达可能与结肠癌的发生、发展、浸润、转移及预后相关,联合评价其功能可能对结肠癌治疗具有一定指导意义。
Objective To investigate the expression of tailed homeobox transcription factor-2 (CDX-2) and tumor suppressor gene KAI-1 in colon cancer and analyze its clinical significance. Methods The expressions of CDX-2 and KAI-1 in 50 cases of colorectal cancer tissues, adjacent paracancerous tissues and 25 cases of normal colonic mucosa tissues were detected by immunohistochemical SP method. The relationship between CDX-2 and KAI-1 protein expression and the clinicopathological features of colon cancer was analyzed. Correlation between the two. Results ① The positive rates of CDX-2 protein expression in colon cancer tissues and corresponding adjacent tissues (≤ 2 cm away from cancer tissues) and normal colonic mucosa were 34% (17/50), 54% (27/50) and The positive rate of KAI-1 protein expression was 30% (15/50), 58% (29/50) and 92% (23/25) in 88% (22/25) The positive rate of protein expression in cancer tissue was significantly lower than that in corresponding paracancerous tissue (P <0.05) and normal colorectal mucosa (P <0.05), and the positive rate in paracancerous tissues was also significantly lower than that in normal colon Mucosal tissue (P <0.05). The positive rate of CDX-2 and KAI-1 protein expression was related to the lymph node metastasis, depth of invasion and differentiation of colon cancer (P <0.05), that is, in patients with lymph node metastasis, deep infiltration depth and serosa and the lower degree of differentiation -2 and KAI-1 protein expression were significantly lower than those without lymph node metastasis, depth of invasion and serosa and the higher degree of differentiation (P <0.05), both with the patient’s age of onset and sex ( P> 0.05). ③ Spearman rank correlation analysis showed that there was a positive correlation between CDX-2 and KAI-1 protein expression (r s = 0.544, P <0.01). Conclusions The expression of CDX-2 and KAI-1 may be related to the occurrence, development, invasion, metastasis and prognosis of colon cancer. To evaluate the function of CDX-2 and KAI-1 may play a guiding role in the treatment of colon cancer.