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目的 研究左旋多巴 (L - dopa)治疗对实验性帕金森病 (PD)大鼠黑质纹状体肿瘤坏死因子(TNF-α)表达的影响。方法 黑质定位注射 6 -羟多巴胺 (6 - OHDA )制备偏侧 PD大鼠模型 ,经 5 0～ 10 0 mg/ kg体重 L - dopa灌胃治疗 4周后 ,检测双侧额叶皮质、黑质和纹状体区域 TNF-α的表达。结果 6 - OHDA损毁侧黑质和纹状体 TNF-α含量和阳性细胞面密度分别显著高于健侧 (均 P<0 .0 5 )。损毁侧与健侧 TNF-α含量的比率随 L - dopa治疗用量的增加而增高 ,且均显著高于对照组 (P<0 .0 5 )。结论 L - dopa治疗进一步加剧了PD大鼠黑质纹状体区域 TNF-α的高表达。
Objective To investigate the effects of L - dopa on the expression of tumor necrosis factor (TNF) in nigrostriatal nuclei in experimental Parkinson ’s disease (PD) rats. Methods PD rats were injected with 6 - hydroxydopamine (6 - OHDA) via the substantia nigra and the rats were treated with L - dopa 50 ~ 100 mg / kg for 4 weeks. The bilateral frontal cortex, The expression of TNF-α in the substantia nigra and striatum regions. Results The content of TNF - α and the density of positive cells in substantia nigra and striatum of 6 - OHDA lesion were significantly higher than that of contralateral side (all P <0.05). The ratio of TNF - α in damaged side and contralateral side increased with the increase of L - dopa treatment dose, which were significantly higher than those in control group (P <0.05). Conclusion L - dopa treatment further aggravates the high expression of TNF-α in nigrostriatal area of PD rats.