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目的探讨HBsAg阳性肝细胞肝癌(HCC)患者乙型肝炎病毒(HBV)DNA基因型与其患HCC之间的关系。方法运用PCR条带分析与基因测序相结合的方法对我院500例HBsAg阳性患者(其中HCC患者150例)的HBV DNA进行分型,并对分型结果进行分析。结果 HBV DNA B型和C型基因型是HBsAg阳性HCC患者和非HCC患者的共同优势基因型,但HCC患者中C型基因型所占比例为65.33%(98/150),明显高于非HCC患者的25.14%(88/350),而B型则相反,分别为28.67%(43/150)与68.86%(241/350),χ2=75.45,P<0.05。HCC患者中,HBV的B型与C型基因型分布在不同性别及不同年龄段之间的差异无统计学意义(P>0.05)。结论 HBV DNA C型基因型在HCC患者中多见,可能与HCC的发生有关。
Objective To investigate the relationship between hepatitis B virus (HBV) DNA genotypes and HCC in patients with HBsAg positive hepatocellular carcinoma (HCC). Methods The HBV DNA of 500 HBsAg positive patients (including 150 with HCC) in our hospital were genotyped by the combination of PCR and gene sequencing. The typing results were analyzed. Results HBV DNA genotypes B and C were the most common genotypes of HBsAg-positive patients and non-HCC patients, but the proportion of C genotypes in HCC patients was 65.33% (98/150), significantly higher than that of non-HCC patients Patients had 25.14% (88/350) compared with 28.67% (43/150) and 68.86% (241/350), respectively, for type B, with χ2 = 75.45, P <0.05. In HCC patients, there was no significant difference in the distribution of HBV genotypes B and C between different genders and different age groups (P> 0.05). Conclusion HBV DNA genotype C is more common in HCC patients and may be related to the occurrence of HCC.