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应用免疫组化技术在３２例肝细胞肝癌及其癌旁肝组织石蜡切片上检测乙型肝炎病毒Ｘ抗原的分布特点，应用免疫电镜进一步观察Ｘ抗原在肝癌细胞内的分布（１１）例）。免疫组化结果显示：肝癌及癌旁肝组织内Ｘ抗原的检出分别为２０／３２（６５．６％）和２５／３２（７８．５％），Ｘ抗原主要分布于肝细胞浆内，常有核周或包膜加强，偶有胞核阳性．免疫电镜示肝癌细胞内Ｘ抗原主要分布于细胞浆和粗面内质网上，少数细胞核内阳性。还对Ｘ抗原的产生及其与肝细胞肝癌的关系进行了讨论。
Immunohistochemical technique was used to detect the distribution of hepatitis B virus X antigen on paraffin sections of 32 cases of hepatocellular carcinoma and paracancerous liver tissues. The distribution of X antigen in hepatoma cells was further observed by immunoelectron microscopy (11 cases). The results of immunohistochemistry showed that the detection of X antigen in HCC and adjacent liver tissues was 20/32 (65.6%) and 25/32 (78.5%) respectively, and X antigen was mainly distributed in the cytoplasm of liver cells. Often nuclear or envelope strengthening, occasionally nuclear positive. Immunoelectron microscopy showed that the X antigen in liver cancer cells mainly distributed in cytoplasm and rough endoplasmic reticulum, and a few cells were positive in the nucleus. The production of X antigen and its relationship with hepatocellular carcinoma have also been discussed.