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目的:研究祖师麻不同部位的香豆素类成分分布。方法:采用HPLC测定不同部位中祖师麻甲素和伞形花内酯的含量,色谱条件:Kromasil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸溶液(15∶85),检测波长326 nm,流速1.0 mL·min-1;采用UV测定不同部位中总香豆素的含量。结果:根皮和老茎皮中祖师麻甲素含量较高,叶中伞形花内酯含量最高,皮部祖师麻甲素和伞形花内酯的含量均高于芯部,总香豆素主要分布在皮部和叶部,其含量是芯部的4~7倍。结论:祖师麻的根皮和老茎皮既是质量较优的部位,又是采收时容易分离获取的部位,祖师麻叶部的药用开发需更深入研究。
Objective: To study the distribution of coumarins in different parts of Patriarch. Methods: HPLC method was used to determine the contents of ephedrine and umbellate in different parts. The chromatographic conditions were as follows: Kromasil C18 column (4.6 mm × 250 mm, 5 μm), mobile phase acetonitrile-0.1% formic acid solution ), The detection wavelength was 326 nm and the flow rate was 1.0 mL · min-1. The content of total coumarins in different parts was determined by UV. Results: The content of ephedrine in root bark and old stem bark was higher, the content of umbelliferone in leaves was the highest, and the contents of epidermes and umbelliferone in epidermis were higher than those in core, Vegetarian mainly in the skin and leaves Department, its content is 4 to 7 times the core. Conclusion: The root bark and old stem bark of Zu Shi Ma is not only the better quality site, but also the easily detachable site when harvesting. The medicinal development of Zu Shi Ma leaf area should be further studied.