以7个籼稻不育系的花药为材料,探讨了籼稻不育系花药培养诱导的条件。结果表明,材料品种对诱导效率的影响最为明显;诱导培养基中激素浓度处理对籼稻花药的愈伤诱导率的影响有显著差异,KT浓度以1 mg/L为宜,碳源以蔗糖50 g/L相对较好,但替换成50 g/L的麦芽糖后诱导效率相对稳定;取材后以8℃低温预处理12 d达到最好效果。 Seven anthers of indica CMS lines were used as materials to investigate the induction conditions of anther culture in indica CMS lines. The results showed that the effect of material variety on induction efficiency was the most obvious. The effects of hormone concentration in induction medium on callus induction rate of indica anther were different. KT concentration was 1 mg / L, carbon source was 50 g / L is relatively good, but the conversion efficiency of 50 g / L maltose is relatively stable; the best effect is obtained after pretreated with 8 ℃ low temperature for 12 days.