研制了一种自然基质河纯毒素阳性质控样品,通过空白河鲀鱼肌肉基质添加河鲍毒素,配方为C18和石墨化碳粉末,此例中添加剂C18和石墨化碳比例为1:1.样品的均匀性和稳定性经过高效液相色谱-串极质谱检测,由6家实验室协作定值并评估.检测结果证明该质控样的特性值具有良好的均稳性,可用于河鲍鱼产品中河纯毒素检测技术评估.“,”A method for preparing tetrodotoxin natural matrix positive quality control sample through spiking the blank muscle of Fugu was conducted,mixed with additives of Cleanert PestiCarb (GCB) and C18 (in this case,mGCB/mC18 =1:1),The homogeneity and stability of the prepared sample were detected by liquid chromatography-tandem mass spectrometry.A cooperative certification was conducted with 6 accredited laboratories.The results showed that the property value in the prepared positive quality control sample had a good homogeneity and stability.The prepared sample could be used for method validation and quality control in determination tetrodotoxin in Fugu products.