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建立了１６ＳｒＲＮＡ基因检测军团菌的多聚酶链反应方法。扩增参考菌株染色体ＤＮＡ（Ｌ．Ｐｎｅｕｍｏｐｈｉｌａ１～１４、Ｌ．ｂｏｚｅｍａｎｎｉ、Ｌ．ｄｕｍｏｆｉ、Ｌ．ｌｏｎｇｂｅａｃｈａｅ、Ｌ．ｍｉｃｄａｄｅｉ、Ｌ．ｊｏｒｄａｎｉｓ），均可检出出３８６ｂｐ的基因片段，敏感性为１０３ｃｆｕ／ｍｌ（平均值）；而扩增９株非军团菌均为阴性。对模拟血标本的检测，其敏感性可达１０２ｃｆｕ／ｍｌ。应用本检测系统检测了２４例临床标本，包括１９份血、５份胸水，阳性率为７０．８％（１７／２４）。与血清学检测和临床诊断治疗结果相符合。上述结果表明该方法敏感、特异、快速、简便。
A 16S rRNA gene was established for the detection of Legionella by polymerase chain reaction. A 386bp gene fragment was amplified with the reference strain chromosomal DNA (L. pneumophila1-14, L. bozemanni, L.dumofi, L. longbeachae, L.micadensis, L.jordanis) with a sensitivity of103cfu / ml (Average), while 9 non-Legionella strains were negative. The simulation of blood tests, the sensitivity of up to 102cfu / ml. Twenty-four clinical specimens, including 19 blood samples and 5 pleural effusions, were detected by this detection system. The positive rate was 70.8% (17/24). Consistent with the results of serological tests and clinical diagnosis. The above results show that the method is sensitive, specific, rapid and easy.