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采用离子交换层析和免疫印迹法分离、纯化和分析血红素和苯肼诱导后大鼠肝脏、脑组织．结果显示：纯化诱导后的大鼠肝脏，获得ＨＯ１和ＨＯ２，前者活性高于后者为２∶１．未诱导的大鼠肝脏仅获得ＨＯ２，但诱导剂作用后，ＨＯ１活性明显增加，而ＨＯ２未见改变．ＨＯ１和ＨＯ２表观分子质量分别为３０ｋｕ和３６ｋｕ．诱导剂未作用的肝脏及作用的脑层析后仅获得ＨＯ２活性的洗脱峰．免疫印迹法检测发现大鼠肝脏ＨＯ２抗体与脑ＨＯ２间有交叉反应，与肝脏ＨＯ１无反应．实验表明在诱导剂作用的大鼠肝脏内含ＨＯ１和ＨＯ２同工酶，其中ＨＯ１为诱导型酶．诱导剂作用的脑仅含ＨＯ２．两种构型在表观分子质量，诱导性和免疫化学特性方面明显不同．
The liver and brain tissues of rats were isolated, purified and analyzed by ion exchange chromatography and immunoblotting. The results showed that: after purification induced rat liver, HO 1 and HO 2, the former activity is higher than the latter 2: 1. Uninduced rat liver only HO 2, but the role of inducer, HO 1 activity was significantly increased, while HO 2 no change. The apparent molecular weights of HO1 and HO2 were 30ku and 36ku, respectively. Inducible agent role in the liver and the role of the brain after only HO 2 activity peak elution. Western blot detection found that rat liver HO 2 antibody and brain HO 2 cross-reaction, and the liver HO 1 no response. Experiments show that the inducer effect in rat livers containing HO 1 and HO 2 isozymes, in which HO 1 is an inducible enzyme. Inducer effect of the brain contains only HO 2. The two configurations differ significantly in apparent molecular mass, inducibility and immunochemical properties.