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目的:通过检测各类型白血病骨髓中RASSF1A基因启动子区甲基化水平,探讨其对白血病分型的临床检测意义。方法:抽选93例不同类型白血病患者(观察组)予以甲基化特异性PCP(MSP)方法进行骨髓RASSF1A基因甲基化状态检测,研究不同类型白血病甲基化状态差异,同期抽选93例非白血病者为对照研究(对照组)。结果:观察组中有13例(13.98%)检测到RASSF1A基因甲基化,而对照组中RASSF1A基因甲基化率为0%,比较差异显著(P<0.05)。不同类型白血病RASSF1A甲基化率比较:淋巴系显著高于髓系(P<0.05),急性与慢性白血病比较差异无显著性(P>0.05)。结论:白血病骨髓中MSP法检测存在RASSF1A甲基化;而RASSF1A基因在淋巴系白血病中的甲基化概率明显增高,因此,对RASSF1A进行甲基化检测有可能作为白血病临床诊断分型的生物学指标之一。
Objective: To detect the methylation level of RASSF1A promoter in bone marrow of all kinds of leukemia and to explore the clinical significance of RASSF1A gene promoter methylation in leukemia typing. Methods: The methylation status of RASSF1A gene in bone marrow of 93 patients with different types of leukemia (observation group) was detected by methylation-specific PCP (MSP) method. The methylation status of different types of leukemia was studied. 93 patients Non-leukemia control study (control group). Results: The methylation of RASSF1A gene was detected in 13 cases (13.98%) in the observation group and 0% in the control group (P <0.05). The methylation rates of RASSF1A in different leukemias were significantly higher in lymphatic system than in myeloid (P <0.05). There was no significant difference between acute and chronic leukemia (P> 0.05). CONCLUSION: The methylation of RASSF1A is detected by MSP assay in leukemia bone marrow. The methylation of RASSF1A gene in lymphoid leukemia is significantly increased. Therefore, methylation detection of RASSF1A might be used as a biomarker for clinical diagnosis of leukemia One of the indicators.