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目的观测急性梗阻性化脓性胆管炎(acute obstructive suppurative cholangitis,AOSC)时大鼠肝组织中清道夫受体-A(scavenger receptor A,SR-A)的表达及其与炎症介质的产生和肝组织损害的关系。方法结扎大鼠胆总管,于胆总管内注入大肠杆菌O111∶B4(菌落浓度5×109cfu/ml),建立急性胆管炎动物模型。测定血浆中内毒素,观测不同时相点肝组织中SR-A mRNA及蛋白质的表达及肝组织病理形态学变化,同时测定TNF-α含量变化,并与结扎胆总管注射生理盐水组、假手术组比较。结果在急性胆管炎组中,随着梗阻感染时间的延长和血浆内毒素浓度的升高,血浆中TNF-α含量明显增加,肝组织中SR-A的mRNA和蛋白质的表达明显下降,与对照组比较有统计学差异(P<0.01)。同时可见随着处理时间延长而逐渐加重的肝组织损伤的病理改变。结论AOSC时,随着肝组织中SR-A表达的下调,枯否细胞(Kupffer cells,KCs)清除、灭活内毒素的能力下降,同时KCs激活释放的细胞因子增多,诱发并加重肝组织的损害。
Objective To observe the expression of scavenger receptor A (SR-A) and its relationship with the production of inflammatory mediators and liver tissue in acute obstructive suppurative cholangitis (AOSC) Damage to the relationship. Methods The common bile duct of rats was ligated and E.coli O111: B4 (colony concentration of 5 × 109 cfu / ml) was injected into the common bile duct to establish acute cholangitis model. The levels of endotoxin in plasma were measured. The expression of SR-A mRNA and protein in liver tissue and histopathological changes of liver tissue were observed at different time points. The changes of TNF-α content were also observed. Ligation with common bile duct normal saline group, Group comparison. Results In the acute cholangitis group, the content of TNF-α in plasma was significantly increased and the expression of SR-A mRNA and protein in liver tissue was significantly decreased with the prolongation of obstructive infection and the increase of plasma endotoxin concentration. Compared with the control There was significant difference between the two groups (P <0.01). At the same time, we can see the pathological changes of liver tissue injury which gradually aggravate with the prolongation of treatment time. Conclusions With the down-regulation of SR-A expression in liver tissue, the ability of Kupffer cells (KCs) to clear and inactivate endotoxin is decreased in AOSC group. Meanwhile, the cytokines released by activation of KCs are increased and induced and aggravated in liver tissues damage.