疣粒野生稻高抗水稻白叶枯病,但是其具体的抗性机制目前仍不清楚。一氧化氮(nitric oxide,NO)是一种重要的信号分子,在植物的抗病反应中起到了重要的作用,然而对于NO是否参与疣粒野生稻对水稻白叶枯病的抗性目前仍缺乏研究。以抗病的疣粒野生稻和感病的水稻品种日本晴为材料,研究了接种白叶枯病菌对叶片病斑、NO含量、NO亚细胞定位和木质部超微结构的影响。结果表明,病菌侵染导致了日本晴叶片呈现枯黄色的干枯斑,疣粒野生稻叶片呈现褐色的凋亡斑,而且野生稻的病斑长度要明显短于日本晴的病斑长度。接种白叶枯病菌后日本晴叶片内NO含量未见明显的变化,而野生稻叶片内NO含量则显著升高,并且大部分的NO定位于导管细胞壁内。进一步通过电镜观察,发现病菌侵染诱导了野生稻叶片导管细胞壁厚度的明显增加。基于这些结果,推测NO参与了疣粒野生稻对白叶枯病的抗性,其功能可能包括诱导导管细胞壁增厚,从而抑制病菌的进一步侵染。 Warty wild rice is highly resistant to bacterial leaf blight, but its specific resistance mechanism is still unclear. Nitric oxide (NO) is an important signaling molecule that plays an important role in plant disease resistance. However, whether NO is involved in the resistance of WILD GRAY TOBACCO Lack of research. The resistant warty wild rice and the susceptible rice variety Nipponbare were used as materials to study the effects of Xanthomonas oryzae on leaf spot, NO content, NO subcellular localization and ultrastructure of xylem. The results showed that the pathogen infection caused the yellowish dry spot of Nipponbare leaves, the brown spot of wild wart and the spot length of wild rice was obviously shorter than that of Nipponbare. After inoculation with Xanthomonas oryzae, NO content in Nipponbare leaf had no significant change, while the content of NO in wild rice leaves was significantly increased, and most of the NO located in the ductal cell wall. Further observation by electron microscopy showed that pathogen infection induced a significant increase in the ductal cell wall thickness of wild rice leaves. Based on these results, it is speculated that NO is involved in the resistance of Warty Wild Rice to bacterial leaf blight, and its function may include inducing thickening of the ductal cell wall, thereby inhibiting further infection by the germ.