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目的：探讨组织蛋白酶Ｄ（Ｃａｔｈ－Ｄ）、肿瘤转移抑制基因表达蛋白（ｎｍ２３－Ｈ１）的表达与食管癌临床病理特点及预后的关系。方法：应用免疫组织化学Ｓ－Ｐ法，以兔抗Ｃａｔｈ－Ｄ、鼠抗ｎｍ２３－Ｈ１抗体标记６０例食管癌和５例正常的食管粘膜。观察不同分化程度和组织类型食管癌的表达情况，并比较其阳性率。结果：癌组织Ｃａｔｈ－Ｄ阳性３６例（６０．０％），ｎｍ２３－Ｈ１阳性３５例（５８．３％）。Ｃａｔｈ－Ｄ的表达与癌组织分化程度、浸润深度、淋巴结转移和预后均无关（Ｐ＞０．０５）；而ｎｍ２３－Ｈ１蛋白的表达则与癌组织分化程度及淋巴结转移有关（Ｐ＜０．０５，Ｐ＜０．０１），与癌组织浸润深度和预后无关（Ｐ＞０．０５）。结论：ｎｍ２３－Ｈ１可作为一种食管癌淋巴结转移的重要生物学标记物，但Ｃａｔｈ－Ｄ、ｎｍ２３－Ｈ１与预后的关系仍需进一步研究。
Objective: To investigate the relationship between the expression of cathepsin D (Cath-D) and tumor metastasis suppressor gene expression protein (nm23-H1) and the clinicopathological features and prognosis of esophageal cancer. METHODS: Immunohistochemical S-P method was used to label 60 cases of esophageal cancer and 5 cases of normal esophageal mucosa with rabbit anti-Cath-D and murine anti-nm23-H1 antibodies. Observe the different degree of differentiation and tissue type of esophageal cancer expression, and compare the positive rate. RESULTS: The positive rate of Cath-D in 36 cases (60.0%) and 35 cases (58.3%) of nm23-H1 were positive. The expression of Cath-D was not related to the degree of differentiation, depth of invasion, lymph node metastasis and prognosis (P>0.05). The expression of nm23-H1 protein was related to the degree of differentiation and lymph node metastasis (P<0. 05, P<0.01), not related to invasion depth and prognosis of cancer tissue (P>0.05). Conclusion: nm23-H1 can be used as an important biological marker for lymph node metastasis of esophageal cancer, but the relationship between Cath-D, nm23-H1 and prognosis still needs further study.