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:【目的】研究胎儿感染乙型肝炎病毒(HBV)与胎盘感染之间的关系及其意义。【方法】用PCR及斑点杂交法检测母血、新生儿静脉血HBVDNA(确定胎儿感染者),用免疫组化方法(LSAB法)检测61例HBV携带者母亲胎盘HBcAg。【结果】①胎儿HBV感染率为2292%(33/144);②61例胎盘HBcAg阳性率为6885%(41/61),胎儿感染组阳性率(8214%)高于对照组(5455%),P<0025;③母血HBeAg/HBVDNA阳性者,胎盘HBcAg阳性率(8205%)高于母血HBeAg/HBVDNA阴性者(4091%),P<0005;④胎盘各种细胞成分中,以与母血直接接触的蜕膜细胞、末梢绒毛合体细胞及EVT(绒毛外滋养细胞)HBcAg阳性率较高(3607%～4918%)。【结论】胎儿感染HBV与胎盘感染有关,胎盘屏障对HBV有一定阻挡作用。
: 【Objective】 To investigate the relationship between fetal infection of hepatitis B virus (HBV) and placental infection and its significance. 【Methods】 HBVDNA in maternal and neonatal blood was detected by PCR and dot blot hybridization. HBcAg was detected in 61 mothers with HBV carriers by immunohistochemistry (LSAB method). 【Results】 ① The rate of HBV infection in fetuses was 2292% (33/144). ② The positive rate of HBcAg in 61 cases of placenta was 6885% (41/61), the positive rate of fetus infection was 8214% (5455%), P <0025; ③The positive rate of HBcAg in placenta (8205%) was higher in maternal HBeAg / HBVDNA positive than in maternal HBeAg / HBVDNA negative (4091%), P <0005; ④In placenta, Direct contact with blood of decidual cells, peripheral villous syncytial cells and EVT (extravillous trophoblast) HBcAg positive rate (3607% ~ 4918%). 【Conclusion】 Fetal infection of HBV is related to placental infection, and placental barrier has a certain block effect on HBV.