利用组织培养技术,在含不同浓度NaCl(0、0.2%、0.4%、0.6%、0.8%、1.0%)的MS增殖及生根培养基上对叶片分化苗进行盐胁迫处理,测定并分析了中华补血草的生长发育指标(株高、叶数、叶宽、植株鲜样质量、根长)和生理指标(MDA、SOD、Pro、Na~+、K~+)变化情况。结果表明:在不同浓度NaCl胁迫下,中华补血草组培苗的生长指标均未表现出明显的盐害症状。其中,根长随NaCl浓度增加而增加,1.0%浓度时主根最长,为对照的3.9倍;株高随NaCl浓度的增加呈先增加后降低然后再增加的趋势,0.6%浓度处理时最低,0.8%浓度处理时最高,1.0%浓度处理时有所下降,但与0.8%浓度处理的差异不显著;植株鲜样质量除0.2%处理与对照差异不显著外,其它处理均显著低于对照且这些处理间的差异不显著;叶数总体上随着盐浓度的增加而减少,1.0%浓度处理的叶数最少,为对照的1/3;叶宽随盐浓度的增加呈先降低后增加的趋势,0.4%浓度处理时叶最窄,0.8%浓度处理时叶最宽,但与1.0%处理的叶宽差异不显著。生理指标的测定结果表明:MDA含量、SOD活性和Pro含量均随NaCl浓度增加呈先增加后降低的趋势。MDA含量在0.6%浓度处理时最高,并显著高于对照,在1.0%浓度处理时最低,且显著低于对照;Pro含量也在0.6%浓度处理时最高,并显著高于对照,在1.0%浓度处理时含量虽有所下降,但也显著高于对照;SOD活性在0.8%浓度处理时最高,其次为1.0%浓度处理,二者间差异显著,且均显著高于对照。Na~+、K~+含量均显著高于对照,且Na~+、K~+含量总体上随NaCl浓度的增加而显著增加,K~+/Na~+比随NaCl浓度的增加出现先降低后增加的现象,在0.8%浓度处理时比值最小,1.0%浓度处理时K+/Na+比值显著增加。综上所述,组培苗可以耐含NaCl为1.0%浓度的盐汁生境,在其受到盐胁迫时,可以通过增加SOD活性和Pro含量以及维持高K~+/Na~+比值来保护细胞膜系统,增强吸水能力,缓解盐害。 The tissue culture technique was used to treat the leaves of differentiated seedlings under salt stress on MS proliferation and rooting medium containing different concentrations of NaCl (0, 0.2%, 0.4%, 0.6%, 0.8%, 1.0%), The growth and development indexes (height, leaf number, leaf width, plant fresh weight, root length) and physiological indexes (MDA, SOD, Pro, Na ~ +, K ~ +) of Limonium were observed. The results showed that under different concentrations of NaCl stress, the growth indexes of Limonium sinense did not show obvious salt damage symptoms. The root length increased with the increase of NaCl concentration, and the longest root at 1.0% concentration was 3.9 times of that of the control. The plant height increased first, then decreased then increased with the increase of NaCl concentration, 0.8% and 0.8%, respectively. However, the difference between the fresh weight and the control was not significant except for the 0.2% treatment, while the other treatments were significantly lower than the control The difference between these treatments was insignificant. The leaf number generally decreased with the increase of salt concentration, and the leaf number at 1.0% concentration was the least, which was 1/3 of the control. The leaf width decreased first and then increased with the increase of salt concentration The trend was the narrowest at 0.4% concentration, the broadest at 0.8% concentration, but not significantly different from 1.0% at 1.0% concentration. The results of physiological indicators showed that MDA content, SOD activity and Pro content increased first and then decreased with the increase of NaCl concentration. The content of MDA was the highest at the 0.6% concentration and the highest at the 1.0% concentration, and the lowest at the concentration of 1.0%. The content of Pro was also the highest at 0.6% Although the concentration decreased, it was also significantly higher than that of the control. The activity of SOD was the highest at the concentration of 0.8%, followed by the concentration of 1.0%. The difference was significant between the two groups. The contents of Na ~ + and K ~ + were significantly higher than those of the control, and the content of Na ~ + and K ~ + increased with the increase of NaCl concentration. The K ~ + / Na ~ + ratio decreased first with the increase of NaCl concentration After the increase, the ratio was the lowest when the concentration was 0.8%, and the ratio of K + / Na + was significantly increased when the concentration was 1.0%. In conclusion, the tissue culture seedlings can tolerate NaCl concentration of 1.0% salt juice habitat, which can protect the cell membrane by increasing the activity of SOD and Pro content and maintaining the ratio of high K + / Na ~ + System to enhance water absorption and alleviate salt damage.