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采用光合细菌和微藻分别作为阳极和阴极接种物构建了双室光微生物燃料电池,考察了氮、磷浓度及阳极处理后的养猪废水对阴极微藻生长的影响,探讨了构建的光微生物燃料电池产电性能及去除养猪废水中COD、氨氮和总磷的效果.结果表明,阴极微藻不仅能利用无机硝态氮和氨氮,而且更喜好有机氮尿素;此外,阴极微藻可适应较高浓度的氮(250 mg·L-1)和磷(64.8 mg·L-1).构建的光微生物燃料电池以养猪废水为基质,外载为1000Ω时,稳定输出电压为161 m V;养猪废水的COD、氨氮及总磷去除率分别为91.8%、90.2%和81.7%.养猪废水经阳极光合细菌处理后培养微藻16 d,藻细胞光密度(OD680)可达3.40,略低于对照BG11培养基.因此,构建的光微生物燃料电池在处理养猪废水产电的同时,可收获微藻实现养猪废水资源化.
The photosynthetic bacteria and microalgae were used as anode and cathode inoculum respectively to construct a dual chamber microbial fuel cell. The effects of nitrogen and phosphorus concentration and piggery wastewater after the anodic treatment on the growth of the cathode microalgae were investigated. The constructed microbe Fuel cell performance and removal of COD, ammonia nitrogen and total phosphorus in pig waste water.The results showed that the negative microalgae can not only use inorganic nitrate nitrogen and ammonia nitrogen, but also prefer organic nitrogen urea; In addition, the cathode microalgae can adapt (250 mg · L-1) and phosphorus (64.8 mg · L-1). The constructed microbial fuel cell based on piggery wastewater was stable with a output voltage of 161 mV ; COD removal rate, ammonia nitrogen removal rate and total phosphorus removal rate of piggery wastewater were 91.8%, 90.2% and 81.7%, respectively. Pigment wastewater was cultured for 16 days by anaerobic photosynthetic bacteria, the OD680 of algal cells reached 3.40, Slightly lower than the control BG11 medium.Therefore, the constructed micro-microbial fuel cell in the treatment of pig waste water production at the same time, harvesting microalgae to achieve pig waste water resource.