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目的建立B-myb mRNA表达量检测的实时荧光定量聚合酶链反应(RT-PCR)系统,检测肝细胞肝癌(HCC)中B-myb的表达并分析其临床意义。方法用实时荧光定量聚合酶链反应技术检测70例HCC患者癌组织、癌旁肝组织及18例正常肝组织中B-myb mRNA的表达情况。结果B-myb mRNA在肝癌组织(0.0375±0.0168)及癌旁肝组织(0.0353±0.0128)中的表达水平明显高于在正常肝组织(0.0265±0.0099)中的表达水平(P<0.05),而在肝癌组织及癌旁肝组织中的表达水平差异无统计学意义(P>0.05)。B-myb在人肝癌组织中的表达与临床分期、肝外转移及术后复发明显有关,而与门静脉癌栓、肿瘤个数、肿瘤直径、血清AFP水平及分化程度无明显关系。结论该方法克服了传统PCR只能定性检测而不能定量检测的缺点,为研究B-myb在HCC中的表达提供了定量方法。B-myb可能与肝癌的发生、发展有关。
Objective To establish a real-time fluorescence quantitative polymerase chain reaction (RT-PCR) system to detect the expression of B-myb mRNA and to detect the expression of B-myb in hepatocellular carcinoma (HCC) and to analyze its clinical significance. Methods The expression of B-myb mRNA in 70 HCC patients, adjacent non-cancerous liver tissues and 18 normal liver tissues was detected by real-time fluorescence quantitative polymerase chain reaction. Results The expression of B-myb mRNA in hepatocellular carcinoma (0.0375 ± 0.0168) and adjacent noncancerous liver tissues (0.0353 ± 0.0128) was significantly higher than that in normal liver tissue (0.0265 ± 0.0099) (P <0.05) There was no significant difference in the expression level between HCC tissues and adjacent liver tissues (P> 0.05). The expression of B-myb in human hepatocellular carcinoma was significantly related to clinical stage, extrahepatic metastasis and postoperative recurrence, but not to portal vein tumor thrombus, tumor number, tumor diameter, serum AFP level and differentiation degree. Conclusion This method overcomes the shortcoming that traditional PCR can only detect qualitatively but not quantitatively, and provides a quantitative method for studying the expression of B-myb in HCC. B-myb may be related to the occurrence and development of liver cancer.