目的探讨香加皮水提液对斑马鱼的发育毒性及不同暴露时间药物对心脏毒性的影响。方法参照经济合作与发展组织推荐的方法,将受精后6小时(6 hour-post-fertilization,6 hpf)胚胎暴露在含有不同浓度香加皮水溶液的24孔板中,分别在24、48、72、96 hpf阶段测定胚胎/幼鱼的自主抽动次数、心率、孵化率、死亡率等指标。随后,使用不同浓度的香加皮水溶液处理发育至48、72、96 hpf的斑马鱼24小时,通过死亡率确定不同暴露阶段对斑马鱼心脏毒性的影响。结果各暴露组24 hpf自主抽动次数增加、48 hpf心率以及72 hpf孵化率显著降低,与正常对照组相比均有显著性差异(P<0.05);24 hpf低浓度给药组胚胎发育迟缓、眼点未发育、头部偏小或未发育,高浓度组发育畸形或停留在体节期;48 hpf胚胎卵黄囊畸形、黑色素生成抑制、尾芽未脱落;72 hpf幼鱼出现身体弯曲、心包水肿、卵黄囊畸形等中毒症状。不同发育阶段斑马鱼急性毒性暴露实验结果表明:香加皮对48、72、96 hpf斑马鱼的半数致死浓度(LC_(50))分别为:0.866 mg/m L、0.693 mg/m L和0.843mg/m L。结论香加皮对斑马鱼具有明显的发育毒性和心脏毒性,且48 hpf斑马鱼非常适合进行心脏毒性药物筛选。 Objective To investigate the developmental toxicity of Xiangkapi water extract to zebrafish and the effects of different exposure time on cardiotoxicity. Methods According to the methods recommended by the Organization for Economic Co-operation and Development, embryos 6 hours-post-fertilization (6 hpf) after fertilization were exposed to 24-well plates containing different concentrations of the aqueous solutions of Xiangkapi at 24, 48, , 96hpf stage determination embryo / juvenile autonomic twitches, heart rate, hatchability, mortality and other indicators. Subsequently, zebrafish, which developed to 48, 72, 96 hpf, were treated with different concentrations of shakugu skin for 24 hours and mortality was used to determine the effects of different exposure stages on zebrafish cardiotoxicity. Results 24 hpf autonomic twitching increased, 48 hpf heart rate and 72 hpf hatching rate significantly decreased in each exposure group compared with the normal control group (P <0.05). In the 24 hpf low-dose group, embryonic development was delayed, Eyes were not developed, the head is small or undeveloped, high concentration group deformity or stay in the intima; 48 hpf yolk sac embryo malformations, inhibition of melanin, tail bud did not fall off; 72 hpf juvenile body bent, pericardium Edema, yolk sac malformations and other symptoms. The experimental results of zebrafish acute toxicity at different developmental stages showed that the LC 50 values ​​of Xiangkapi at 48, 72, and 96 hpf were 0.866 mg / m L, 0.693 mg / m L and 0.843 mg / m L. Conclusion Xiangkapi has obvious developmental toxicity and cardiotoxicity to zebrafish, and 48 hpf zebrafish is very suitable for cardiotoxic drug screening.