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目的研究放射线照射后NK92细胞对人卵巢癌细胞杀伤活性及其成瘤性的影响。方法应用医用电子直线加速器对NK92细胞进行照射处理(800cGy),以体外培养的人卵巢癌细胞株HO8910为靶细胞,以细胞株K562作为对照,应用4h51Cr释放法检测不同效靶比情况下体外培养NK92细胞的杀伤活性。建立人卵巢癌SCID鼠肿瘤模型,观察输注经照射后的NK92细胞对荷瘤鼠的治疗效果。结果(1)体外实验NK92细胞未照射组,效靶比较低时(11、51)对HO8910细胞的杀伤率为39%～45%,随效靶比升高,杀伤率上升,101时杀伤率升为59%,201时杀伤率仅上升了6%;对于K562细胞,杀伤率在58%～71%之间。照射后2d,800cGy组NK92细胞对HO8910细胞不同效靶比的杀伤率比0cGy组略有降低,总体杀伤率在33%～58%之间;对于K562细胞,杀伤率在39%～49%之间。照射后NK92细胞数量进行性下降,第5天细胞死亡;(2)动物实验分别在接种后30、40、50d观察治疗组与对照组肿瘤体积,结果显示,分别减小了78%、56%、20%(P<0.01)。结论NK92细胞对人卵巢癌细胞具有较高的杀伤活性,经放射线照射后仍保留一定的杀伤活性,作为一种生物治疗手段治疗卵巢癌行之有效。
Objective To study the effects of irradiation on the killing activity of NK92 cells and the tumorigenicity of human ovarian cancer cells. Methods NK92 cells were irradiated by medical electron linear accelerator (800cGy). The human ovarian cancer cell line HO8910 was cultured in vitro. The cell line K562 was used as a control. The 4h51Cr release assay was used to detect the effect of different target ratios in vitro NK92 cell killing activity. To establish a human ovarian cancer SCID mouse model of tumor, observe the therapeutic effect of irradiated NK92 cells on tumor-bearing mice. Results (1) NK92 cells in vitro irradiation group, the target efficiency is low (11,51) on the killing rate of HO8910 cells was 39% to 45%, with the target ratio increased, the killing rate increased, the killing rate of 101 Rose to 59%, 201 kill rate increased only 6%; for K562 cells, killing rate of 58% to 71%. On the 2nd day after irradiation, the lethality ratio of NK92 cells in 800cGy group to HO8910 cells was slightly lower than that in 0cGy group, and the overall killing rate was between 33% -58%; for K562 cells, the killing ratio was 39% -49% between. The number of NK92 cells decreased progressively after irradiation, and the cells died on the 5th day. (2) The tumor volume of the treated group and the control group were observed at the 30th, 40th and 50th day after inoculation. The results showed that the percentage of NK92 cells decreased by 78%, 56% , 20% (P <0.01). Conclusion NK92 cells have high cytotoxic activity on human ovarian cancer cells, and still retain some cytotoxic activity after irradiation. Therefore, NK92 cells can be used as a biological treatment for ovarian cancer.