我们应用聚合酶链反应一单链构像多态（PCR—SSCP）银染技术对31例非小细胞肺癌（NSCLC）中P53基因第5、6、7、8、外显子进行检测，结果发现有15例（48．38％）发生基因突变，其中第7外显子8例，第5、8外显子各3例，第6外显子1例。说明5～8外显子为突变热点区域；PCR—SSCP银染技术是一种敏感、快迅、经济、实用检测点突变方法。 We used polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) silver staining technique to detect the 5th, 6th, 7th, 8th, and exons of P53 gene in 31 cases of non-small cell lung cancer (NSCLC). Gene mutations were found in 15 cases (48.38%), among which there were 8 exon 7 exons, 3 Exon 5 exons and 8 exons, and exon 6 exon 1 cases. It shows that exon 5-8 is the mutation hotspot region; PCR-SSCP silver staining technology is a sensitive, rapid, economic, and practical detection point mutation method.